Electrophoresis: Principles, Techniques, and Applications in Chemistry
Electrophoresis Questions and Answers: Things You Need to Know About Gel Electrophoresis 1.)What is electrophoresis and how does it work? Answer: Electrophoresis is a technique used to separate charged particles, such as molecules or ions, in an electric field. It works based on the principle that charged particles migrate towards oppositely charged electrodes. The movement of particles is influenced by their charge, size, and shape, allowing for their separation. 2. Explain the principle of gel electrophoresis . Answer: Gel electrophoresis is based on the principle of separating charged molecules, such as DNA fragments or proteins, based on their size and charge. The technique involves loading the samples onto a gel matrix, typically agarose or polyacrylamide, and applying an electric field. Smaller molecules migrate faster through the gel, resulting in distinct bands that can be visualized and analyzed. 3. What is SDS-PAGE and how is it used in electrophoresis ? Answer: SDS-PAGE sta